What is the catalog number for GAPDH Antibody?

The catalog number for GAPDH Antibody is orb1247972.

What sizes are available for GAPDH Antibody?

GAPDH Antibody is available in the following size(s): 0.1 mg.

Is GAPDH Antibody in stock?

GAPDH Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

GAPDH Antibody

Product Specifications

Product Name Alternative

GAPDH, glyceraldehyde-3-phosphate dehydrogenase, HGNC:4141, G3PD, GAPD, MGC88685, OTTHUMP00000174431, OTTHUMP00000174432, aging-associated gene 9 protein, glyceraldehyde 3-phosphate dehydrogenase

UniProt

P04406

Reactivity

Human, Mouse, Rat

Immunogen

The immunogen for this antibody is: C-HQVVSSDFNSDT

Target

GAPDH

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Metabolism Research

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 37kDa band observed in lysates of cell lines Hek293 and HeLa and in Human Liver, Tonsil, Mouse Spleen, and Rat Brain and Spleen lysates (calculated MW of 36.1kDa according to Human NP_002037.2 and 35.8kDa according to Mouse NP_032110.1 and Rat NP_058704.1) . Recommended concentration: 0.01-0.5μg/mL. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 2.5μg/mL.Immunofluorescence: Expression of the protein seen in the cytoplasm and vesicles of HeLa cells. Recommended concentration: 10μg/mL.

Prediction Reactivity

Canine, Porcine

Tested Applications

ELISA, IF, IHC, WB

NCBI Accession Number

NP_002037.2

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.