LEF1 Antibody
LEF1 Antibody
Product Specifications
Product Name Alternative
LEF1, lymphoid enhancer-binding factor 1 , HGNC:6551, DKFZp586H0919, TCF1ALPHA , lymphoid enhancer binding factor-1
UniProt
Q9UJU2
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-QHEQRKEQEPKRPH
Target
LEF1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 50kDa band observed in MOLT4 lysates (calculated MW of 44.2kDa according to NP_057353.1) . The observed molecular weight corresponds to earlier findings in literature (Yasumoto et al , EMBO J. 2002 Jun 3;21 (11) :2703-14.; PMID: 12032083) . Recommended concentration: 1-3μg/mL. Primary incubation was 1 hour.
Prediction Reactivity
Canine, Mouse, Rat
Tested Applications
ELISA, WB
NCBI Accession Number
NP_057353.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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