RXRA Antibody

Product Specifications

Product Name Alternative

RXRA, NR2B1, retinoid X receptor, alpha, FLJ16020, FLJ16733, MGC102720, FLJ00280, FLJ00318

UniProt

P19793

Reactivity

Human

Immunogen

The immunogen for this antibody is: CQVNSSLTSPTGRGSM

Target

RXRA

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 60kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 50.8kDa according to NP_002948.1) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Solomon et al, J Biol Chem. 1998 Jul 10;273 (28) :17573-8; PMID: 9651351. Recommended concentration: 0.3-1μg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful on NIH3T3 cell line for this particular batch.

Prediction Reactivity

Mouse, Rat

Tested Applications

ELISA, WB

NCBI Accession Number

NP_002948.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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