TRAF2 Antibody
TRAF2 Antibody
Product Specifications
Product Name Alternative
TRAF2, TNF receptor-associated factor 2 , MGC:45012, TRAP, TRAP3 , OTTHUMP00000064745, tumor necrosis factor type 2 receptor associated protein 3
UniProt
Q12933
Reactivity
Human, Mouse
Immunogen
The immunogen for this antibody is: C-KMEAKNSYVRDD
Target
TRAF2
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 50kDa band observed in Human Ovary and Mouse Testis lysates and in lysates of cell lines HepG2 and Jurkat. Preliminary testing also showed a band at approx 50kDa in U937, NIH3T3 and HEK293 cell lysates (calculated MW of 55.9kDa; according to Human NP_066961.2) . This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.5μg/ml. Primary incubation 1 hour at room temperature. IHC: Paraffin embedded Human Pancreas, Kidney and Placenta. Recommended concentration: 3.75μg/ml. Immunofluorescence: Strong expression of the protein seen in the endoplasmic reticulums of HeLa and A431 cells. Recommended concentration: 10μg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10μg/mL.
Prediction Reactivity
Bovine, Canine, Rat
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_066961.2
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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