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TRAF2 Antibody

TRAF2 Antibody

Product Specifications

Product Name Alternative

TRAF2, TNF receptor-associated factor 2 , MGC:45012, TRAP, TRAP3 , OTTHUMP00000064745, tumor necrosis factor type 2 receptor associated protein 3

UniProt

Q12933

Reactivity

Human, Mouse

Immunogen

The immunogen for this antibody is: C-KMEAKNSYVRDD

Target

TRAF2

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 50kDa band observed in Human Ovary and Mouse Testis lysates and in lysates of cell lines HepG2 and Jurkat. Preliminary testing also showed a band at approx 50kDa in U937, NIH3T3 and HEK293 cell lysates (calculated MW of 55.9kDa; according to Human NP_066961.2) . This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.5μg/ml. Primary incubation 1 hour at room temperature. IHC: Paraffin embedded Human Pancreas, Kidney and Placenta. Recommended concentration: 3.75μg/ml. Immunofluorescence: Strong expression of the protein seen in the endoplasmic reticulums of HeLa and A431 cells. Recommended concentration: 10μg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10μg/mL.

Prediction Reactivity

Bovine, Canine, Rat

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

NP_066961.2

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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