SFN Antibody
SFN Antibody
Product Specifications
Product Name Alternative
SFN, 14-3-3sigma, stratifin, HGNC:10773, YWHAS
UniProt
P31947
Reactivity
Human, Mouse, Rat
Immunogen
The immunogen for this antibody is: C-DLHTLSEDSYKDST
Target
SFN
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 26-28kDa band observed in Human, Mouse and Rat Skin lysates and in lysates of cell lines A431 and U2OS (calculated MW of 27.8kDa according to Human NP_006133.1, Mouse NP_061224.2 and Rat XP_003750096.1) . Recommended concentration: 0.03-0.1μg/mL. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Skin shows strong nuclear staining in selected cells of the basal layer. Recommended concentration, 2-4μg/mL.
Prediction Reactivity
Canine, Porcine
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_006133.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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