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AIF1 Antibody

AIF1 Antibody

Product Specifications

Product Name Alternative

AIF1, IBA1, allograft inflammatory factor 1 , DASS-82G15.2, AIF-1, IRT-1, Em:AF129756.17, interferon gamma responsive transcript, ionized calcium-binding adapter molecule

UniProt

P55008

Reactivity

Human, Mouse

Immunogen

The immunogen for this antibody is: C-NKQFLDDPKYSSDED

Target

AIF1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Musculoskeletal & Connective Tissue Research

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 17kDa band observed in Human Brain (Frontal Cortex) lysate (calculated MW of 16.7kDa according to NP_001614.3) . Recommended concentration: 0.5-1.5μg/ml. Primary incubation 1 hour at room temperature. IHC: In paraffin embedded Mouse Brain shows staining of astrocytes. Recommended concentration: 2-4μg/ml. Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10μg/mL.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of CaCo-2 cells. Recommended concentration: 10μg/ml.

Prediction Reactivity

Canine

Tested Applications

ELISA, FC, IF, IHC, WB

NCBI Accession Number

NP_001614.3

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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