ABHD5 Antibody
ABHD5 Antibody
Product Specifications
Product Name Alternative
ABHD5, CGI58 protein, aCGI58, bhydrolase domain containing 5, CDS, IECN2, MGC8731, NCIE2
UniProt
Q8WTS1
Reactivity
Human, Mouse
Immunogen
The immunogen for this antibody is: C-FPERPDLADQDR
Target
ABHD5
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Metabolism Research
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 40kDa band observed in lysates of cell lines NIH3T3/3TL (calculated MW of 39.1kDa according to NP_057090.2) . Additional faint bands of 26kDa and 18kDa were also observed, and were successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.2-0.6μg/mL Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Pancreas and Kidney. Recommended concentration: 3.75μg/mL.Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm/ vesicles of U2OS cells. Recommended concentration: 10μg/mL.
Prediction Reactivity
Canine, Rat
Tested Applications
ELISA, IF, IHC, WB
NCBI Accession Number
NP_057090.2
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
orb1669033-01 48 Tests
Rat Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 (HCN3) ELISA Kit
Sign In for Pricing
View Details orb1669033-02 96 Tests
Rat Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 (HCN3) ELISA Kit
Sign In for Pricing
View Details 