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ACACB Antibody

ACACB Antibody

Product Specifications

Product Name Alternative

ACACB, acetyl-Coenzyme A carboxylase beta , ACCB, HACC275 , acetyl-CoA carboxylase 2

UniProt

O00763

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-QLGEPDLSDKDRKD

Target

ACACB

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Metabolism Research

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 28kDa band in Human Heart lysates after 0.5μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 277kDa according to NP_001084.2. The 28kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant ACACB captured by a rabbit antibody. Recommended reporter concentration: 1-2μg/mL

Tested Applications

EIA, ELISA

NCBI Accession Number

NP_001084.2

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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