ACACB Antibody
ACACB Antibody
Product Specifications
Product Name Alternative
ACACB, acetyl-Coenzyme A carboxylase beta , ACCB, HACC275 , acetyl-CoA carboxylase 2
UniProt
O00763
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-QLGEPDLSDKDRKD
Target
ACACB
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Metabolism Research
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 28kDa band in Human Heart lysates after 0.5μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 277kDa according to NP_001084.2. The 28kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant ACACB captured by a rabbit antibody. Recommended reporter concentration: 1-2μg/mL
Tested Applications
EIA, ELISA
NCBI Accession Number
NP_001084.2
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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