AGRP Antibody
AGRP Antibody
Product Specifications
Product Name Alternative
AGRP, ART, AGRT, ASIP2, agouti related protein homolog (mouse), agouti (mouse) related protein, Agouti-related transcript, mouse, homolog of, MGC118963, agouti related protein homolog
UniProt
O00253
Reactivity
Human
Immunogen
The immunogen for this antibody is: CRKLGTAMNPCSRT
Target
AGRP
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave bands at approx 45kDa and 24kDa in Human Brain lysates after 0.1μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 14.4kDa according to NP_001129.1and NP_015531.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) .Immunohistochemistry:Frozen section of Human Hypothalamus shows staining of a group of neuronal cell bodies in the human infundibular (arcuate) nucleus and their varicose axons. Recommended concentration: 0.05-0.1μg/mL.
Prediction Reactivity
Canine, Porcine
Tested Applications
ELISA, IHC
NCBI Accession Number
NP_015531.1, NP_001129.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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