What is the catalog number for HSP90B1 Antibody?

The catalog number for HSP90B1 Antibody is orb1247829.

What sizes are available for HSP90B1 Antibody?

HSP90B1 Antibody is available in the following size(s): 0.1 mg.

Is HSP90B1 Antibody in stock?

HSP90B1 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

HSP90B1 Antibody

Product Specifications

Product Name Alternative

TRA1, GRP94, tumor rejection antigen (gp96) 1 , HGNC:12028, ECGP, GP96, Tumor rejection antigen-1 (gp96), endothelial cell (HBMEC) glycoprotein, glucose regulated protein, 94 kDa, tumor rejection antigen (gp96) 1

UniProt

P14625

Reactivity

Human, Mouse

Immunogen

The immunogen for this antibody is: C-KEGVKFDESEKTKE

Target

HSP90B1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 100kDa band observed in lysates of cell lines HEK293, A549, HeLa, U2OS and NIH3T3 and in preliminary testing of MCF7 and HepG2 cell lysate (calculated MW of 92.5kDa according to Human NP_003290.1 and Mouse NP_035761.1) . This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.3μg/ml. Primary incubation 1 hour at room temperature. IHC: Paraffin embedded Human Uterus and Liver. Recommended concentration: 3-5μg/ml. Immunofluorescence: Strong expression of the protein seen in the ER and cytoplasm of A431 and HeLa cells. Recommended concentration: 10μg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10μg/mL.

Prediction Reactivity

Bovine, Canine, Porcine, Rat, Zebrafish

Tested Applications

ELISA, FC, IF, IHC, WB

NCBI Accession Number

NP_003290.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.