AIRE Antibody

AIRE, autoimmune regulator (automimmune polyendocrinopathy candidiasis ectodermal dystrophy), APS1, APSI, PGA1, APECED, AIRE protein, autoimmune regulator (APECED protein), autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy), autoimmune regulator, AIRE1, OTTHUMP00000109529, autoimmune polyendocrinopathy candidiasis ectodermal dystrophy

Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary testing showed an approx 60kDa band in nuclear lysates of cell line Jurkat and in Human Spleen lysates (calculated MW of 57.7kDa according to NP_000374.1) . Recommended concentration: 1-2μg/mL. Primary incubation 1 hour at room temperature. Negative Control: Human Smooth Muscle lysate. This product has been successfully used in Western blot by an anonymous customer on HEK293 cell lysate.Immunohistochemistry:In paraffin embedded Human Thymic medula shows staining of select nuclei. Recommended concentration: 2.5μg/mL.Immunofluorescence: Strong expression of the protein seen in the nucleoplasm and nuclear membranes of U2OS and HeLa cells. Recommended concentration: 10μg/mL. Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10μg/mL.

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.