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APBB1 Antibody

APBB1 Antibody

Product Specifications

Product Name Alternative

APBB1, FE65, amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65), MGC:9072, RIR , adaptor protein FE65a2, amyloid beta A4 precursor protein-binding, family B, member 1, stat-like protein

UniProt

O00213

Reactivity

Mouse

Immunogen

The immunogen for this antibody is: C-GSLKPKRLGAHTP

Target

APBB1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 100kDa band observed in lysates of NIH/3T3 (calculated MW of 77.2kDa according to NP_001155.1 and 77.0kDa according to NP_663722.1) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Zambrano et al, J Biol Chem. 1998 Aug 7;273 (32) :20128-33.; PMID: 9685356) . Recommended concentration: 0.1-0.3μg/mL. Primary incubation was 1 hour.

Prediction Reactivity

Human

Tested Applications

ELISA, WB

NCBI Accession Number

NP_001155.1, NP_663722.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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