SLC7A11 Antibody
SLC7A11 Antibody
Product Specifications
Product Name Alternative
SLC7A11, solute carrier family 7, (cationic amino acid transporter, y+ system) member 11, CCBR1, xCT , cystine/glutamate transporter
UniProt
Q9UPY5
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-KGQTQNFKDAFSGRD
Target
SLC7A11
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 50-55kDa band observed in lysates of cell lines A549 and U2OS, and in Human Smooth Muscle and Tonsil lysates (calculated MW of 55.4kDa according to NP_055146.1) . Recommended concentration: 0.03-0.3μg/mL. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Placenta and Kidney. Recommended concentration: 3.75μg/mL.Immunofluorescence: Strong expression of the protein seen in the vesicles/ cytoplasm of A549 cells. Recommended concentration: 10μg/mL. Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10μg/mL.
Tested Applications
ELISA, FC, IF, IHC, WB
NCBI Accession Number
NP_055146.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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