BCL7A Antibody

Product Specifications

Product Name Alternative

BCL7A, BCL7, B-cell CLL/lymphoma 7A, B-cell CLL/lymphoma-7

UniProt

Q4VC05

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-MKLEASQQNSEEM

Target

BCL7A

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 26kDa band observed in Human Lymph Node lysates (calculated MW of 25.0kDa according to NP_066273.1) . Recommended concentration: 1-3μg/mL. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10μg/mL. Flow Cytometry: Flow cytometric analysis of Daudi cells. Recommended concentration: 10μg/mL.

Prediction Reactivity

Bovine, Canine, Mouse, Porcine, Rat

Tested Applications

ELISA, FC, IF, WB

NCBI Accession Number

NP_066273.1, NP_001019979.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.