C14orf68 Antibody
C14orf68 Antibody
Product Specifications
Product Name Alternative
C14orf68, chromosome 14 open reading frame 68 , HDMCP, HMFN1655 , hepatocellular carcinoma-downregulated mitochondrial carrier protein
UniProt
Q6Q0C1
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-RYGNPDAKPTKAD
Target
C14orf68
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 45kDa band in Human Liver lysates after 0.3μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 33.4kDa according to NP_997000.2. The 45kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Liver shows staining of nucleoli in select hepatocytes. Recommended concentration, 2.5μg/mL.
Tested Applications
ELISA, IHC
NCBI Accession Number
NP_997000.2
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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