CCKBR Antibody

Product Specifications

Product Name Alternative

CCKBR, cholecystokinin B receptor , HGNC:1571, CCK-B, GASR, CCK2 receptor, cholecystokinin-B receptor/gastrin receptor, gastrin receptor, gastrin\cholecystokinin brain receptor, CCK2R

UniProt

P32239

Reactivity

Human, Mouse, Rat

Immunogen

The immunogen for this antibody is: C-FDGDSDSDSQSRVRNQ

Target

CCKBR

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:16000. Western Blot: Approx 80kDa band observed in Mouse Pancreas and Rat Stomach lysates (calculated MW of 49.2kDa according to Mouse NP_031653.1 and 49.0kDa according to Rat NP_037297.1) . The observed molecular weight also corresponds to earlier findings in literature with different antibodies. Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour. Immunohistochemistry: Paraffin embedded Human Uterus. Recommended concentration: 5ug/ml. In paraffin embedded Human Pancreas expression of CCKBR is detected in islet cells, and not in surrounding acinar cells. Recommended concentration: 6ug/ml.

Prediction Reactivity

Bovine

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

NP_795344.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.