CXCR3 Antibody

CXCR3, chemokine (C-X-C motif) receptor 3, HGNC:4540, CD183, CKR-L2, CMKAR3, GPR9, IP10, IP10-R, Mig-R, MigR, G protein-coupled receptor 9, IP10 receptor, Mig receptor, chemokine (C-X-C) receptor 3

Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments showed a band at approx 55-60kDa in Human Tonsil and Mouse Spleen lysates after 1μg/mL antibody staining. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature. Please note we cannot currently find an explanation in the literature for this band, given the calculated size of 45.5kDa according to NP_001136269.1Immunofluorescence: Strong expression of the protein seen in the membranes of A431 and HepG2 cells. Recommended concentration: 10μg/mL. Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10μg/mL.

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.