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DDX6 Antibody

DDX6 Antibody

Product Specifications

Product Name Alternative

DEAD-box protien 6, DEAD (Asp-Glu-Ala-Asp) box polypeptide 6, DDX6, HLR2, P54, RCK, DEAD box-6, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD)

UniProt

P26196

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-AEYHSEPVEDEKP

Target

DDX6

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 50kDa band observed in lysates of cell lines Daudi, Jurkat and HepG2 (calculated MW of 53.2kDa according to NP_004388.1) . Recommended concentration: 0.3-1μg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cortex) and Thymus. Recommended concentration: 5μg/mL.

Prediction Reactivity

Canine, Mouse, Rat

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

NP_004388.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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