What is the catalog number for DARC Antibody?

The catalog number for DARC Antibody is orb1247588.

What sizes are available for DARC Antibody?

DARC Antibody is available in the following size(s): 0.1 mg.

Is DARC Antibody in stock?

DARC Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

DARC Antibody

Product Specifications

Product Name Alternative

FY, DARC, HGNC:4035, Duffy blood group, CCBP1, GPD, CTA-134P22.3, CD234, FY, OTTHUMP00000060081, Duffy blood group, chemokine receptor, Duffy blood group antigen, Glycoprotein D, Dfy, GpFy, WBCQ1, Fy glycoprotein, OTTHUMP00000035293, OTTHUMP00000035294

UniProt

Q16570

Reactivity

Human

Immunogen

The immunogen for this antibody is: HRAELSPSTENSSQLDFED-C

Target

DARC

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 37kDa band observed in Human Liver lysates (calculated MW of 35.6kDa according to NP_002027.2) . Recommended concentration: 0.3-1μg/mL. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Kidney stains epithelial cells of renal tubules and endothelial cells of vessels. The granular staining is compatible with the endoplasmic reticulum association of the protein. Recommended concentration: 3μg/mL. Paraffin embedded Human Lung. Recommended concentration: 5μg/mL.Flow cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10μg/mL. A customer has reported successful staining of HEK293S-TetR transfected with DARC receptor at a dilution of 1/1000.

Tested Applications

ELISA, FC, IHC, WB

NCBI Accession Number

NP_001116423.1, NP_002027.2

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.