DYX1C1 Antibody
DYX1C1 Antibody
Product Specifications
Product Name Alternative
DYX1C1, DYX1, EKN1, DYXC1, FLJ37882, dyslexia susceptibility 1 candidate 1, MGC70618, RD
UniProt
Q8WXU2
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-KIRNVIQGTELKS
Target
DYX1C1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Neuroscience
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Lysates of COS1 transfected with full length recombinant Human DYX1C1 gave bands at approx 48kDa after 0.1μg/mL antibody staining. In addition, a minor band of 24kDa is detected consistent with observations with N-terminal specific antibodies (unpublished data, LoTurco, Connecticut, USA) . The 75kDa band is visible in the non-transfected COS1 also (first lane) and is therefore non-specific. We call for caution when this product is used in other assays than Western blot. Data kindly provided by Wang and LoTurco, University of Connecticut, USA.Immunohistochemistry:In paraffin embedded Human Cerebral Cortex shows cytoplasm staining in some of the neuronal cells. Recommended concentration, 5-10μg/mL.
Prediction Reactivity
Mouse, Rat
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_570722.2
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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