ACSL4 Antibody

Product Specifications

Product Name Alternative

ACSL4, acyl-CoA synthetase long-chain family member 4, FACL4, fatty-acid-Coenzyme A ligase, long-chain 4, ACS4, LACS 4, lignoceroyl-CoA synthase, Fatty acid coenzyme A ligase, long-chain 4, MRX63, LACS4, MRX68, acyl-CoA synthetase 4, long-chain fatty-acid-Coenzyme A ligase 4

UniProt

O60488

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-HYLKDIERMYGGK

Target

ACSL4

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Metabolism Research

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 80kDa band observed in lysates of cell line HepG2 (calculated MW of 79.2kDa according to NP_075266.1) . Recommended concentration: 0.1-0.3μg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Small Intestine and Brain (Cortex) . Recommended concentration: 3.75μg/mL.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Prediction Reactivity

Bovine, Canine, Mouse, Porcine, Rat

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

NP_075266.1, NP_004449.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.