FHL1 Antibody
FHL1 Antibody
Product Specifications
Product Name Alternative
FHL1, SLIM1, KYO-T, RP11-535K18.1, FHL1A, four and a half LIM domains 1, Four-and-a-half LIM domains 1, skeletal muscle LIM-protein1, FHL1B, KYOT, MGC111107, XMPMA, SLIMMER, LIM protein SLIMMER, four-and-a-half Lin11, Isl-1 and Mec-3 domains 1, skeletal muscle LIM-protein1
UniProt
Q13642
Reactivity
Human, Mouse
Immunogen
The immunogen for this antibody is: CNKRFVFHNEQVY
Target
FHL1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Stem Cell & Developmental Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 30kDa band observed in Human Muscle lysates (calculated MW of 31.9kDa according to NP_001440) . Recommended concentration: 0.05-0.3μg/mL.Immunohistochemistry:In paraffin embedded Human Testis shows fibrous staining in the cytoplasm of developing sperm cells. Recommended concentration: 2μg/mL.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Prediction Reactivity
Bovine, Canine, Porcine, Rat
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_001440.2, NP_001153171.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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