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FHL1 Antibody

FHL1 Antibody

Product Specifications

Product Name Alternative

FHL1, SLIM1, KYO-T, RP11-535K18.1, FHL1A, four and a half LIM domains 1, Four-and-a-half LIM domains 1, skeletal muscle LIM-protein1, FHL1B, KYOT, MGC111107, XMPMA, SLIMMER, LIM protein SLIMMER, four-and-a-half Lin11, Isl-1 and Mec-3 domains 1, skeletal muscle LIM-protein1

UniProt

Q13642

Reactivity

Human, Mouse

Immunogen

The immunogen for this antibody is: CNKRFVFHNEQVY

Target

FHL1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Stem Cell & Developmental Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 30kDa band observed in Human Muscle lysates (calculated MW of 31.9kDa according to NP_001440) . Recommended concentration: 0.05-0.3μg/mL.Immunohistochemistry:In paraffin embedded Human Testis shows fibrous staining in the cytoplasm of developing sperm cells. Recommended concentration: 2μg/mL.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Prediction Reactivity

Bovine, Canine, Porcine, Rat

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

NP_001440.2, NP_001153171.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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