GATA1 Antibody

GATA1, GATA binding protein 1 (globin transcription factor 1), HGNC:4170, ERYF1, GF1, NFE1, GATA binding protein 1, GATA-binding protein 1 (globin transcription factor 1), erythrold transcription factor 1, globin transcription factor 1, transcription factor GATA1, GF-1, XLTT, NF-E1 DNA-binding protein, erythroid transcription factor 1

Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 50-55kDa band observed in nuclear lysates of cell line K562 calculated MW of 42.8kDa according to NP_002040.1) . The observed molecular weight corresponds to different antibodies from other commercial sources. Recommended concentration: 0.3-1μg/mL. Primary incubation 1 hour at room temperature. Negative Control: Human Hippocampus lysate.Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and NIH3T3 cells. Recommended concentration: 10μg/mL. Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10μg/mL.

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.