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CCNDBP1 Antibody

CCNDBP1 Antibody

Product Specifications

Product Name Alternative

CCNDBP1, cyclin D-type binding-protein 1, DIP1, GCIP, HHM Protein, MAID protein, grap2 cyclin interacting protein, D-type cyclin-interacting protein 1

UniProt

O95273

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-NRIKELTQSELEL

Target

CCNDBP1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 55kDa band in K562 lysate at 0.5μg/mL. This band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 41kDda and 27kDa according to NP_036274; NP_411241. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Prostate shows textured cytoplasm staining with emphasis on the luminal side of the secretory cells in the glands. Recommended concentration, 3-6μg/mL.

Prediction Reactivity

Canine

Tested Applications

ELISA, IHC

NCBI Accession Number

NP_411241.1, NP_036274.3

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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