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GOLGA3 Antibody

GOLGA3 Antibody

Product Specifications

Product Name Alternative

GOLGA3, HGNC:4426, GCP170, MEA-2 , Golgi autoantigen, golgin subfamily a, 3, Golgi complex-associated protein of 170 kD, Golgi membrane associated protein, Golgi peripheral membrane protein, SY2/SY10 protein, golgin-160, golgin-165, male enhanced antigen-2

UniProt

Q08378

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-HSQSRASKEGPGE

Target

GOLGA3

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 170kDa band observed in human HeLa lysates (calculated MW of 167kDa according to NP_005886.2) . Recommended concentration: 0.1-0.3μg/mL. An additional band of unknown identity was also consistently observed at appox 25kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Tested Applications

ELISA, WB

NCBI Accession Number

NP_005886.2

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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