GOT1 Antibody
GOT1 Antibody
Product Specifications
Product Name Alternative
GOT1, glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1), GIG18, aspartate aminotransferase 1, growth-inhibiting protein 18
UniProt
P17174
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-RSYRYWDAEKR
Target
GOT1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 40kDa band observed in Human Brain (Cerebellum), Liver and Skeletal Muscle lysates (calculated MW of 46.2kDa according to NP_002070.1) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Berk et al, Proc Natl Acad Sci U S A. 1990 May;87 (9) :3484-8; PMID: 2185471) . Recommended concentration: 0.01-1μg/mL.Immunohistochemistry:In paraffin embedded Human Heart shows strong staining of the capillary network between the myocardial fibres in transverse secion. Recommended concentration: 2-3μg/mL. Paraffin embedded Human Kidney. Recommended concentration: 5μg/mL.
Prediction Reactivity
Rat
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_002070.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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