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GPX3 Antibody

GPX3 Antibody

Product Specifications

Product Name Alternative

GPX3, glutathione peroxidase 3 (plasma), GPx-P, GSHPx-3, GSHPx-P , extracellular glutathione peroxidase, plasma glutathione peroxidase 3

UniProt

P22352

Reactivity

Human, Mouse, Porcine

Immunogen

The immunogen for this antibody is: C-NQFGKQEPGENSE

Target

GPX3

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Metabolism Research

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 26kDa band observed in Mouse Kidney and Liver lysates (calculated MW of 27.7kDa according to Mouse NP_001077398.1 [isoform1], 25.4kDa according to Mouse NP_032187.2 [isoform 2] and 25.5kDa according to Human NP_002075.2) . Recommended concentration: 1-3μg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Placenta and Colon. Recommended concentration: 5μg/mL. Frozen sections of Porcine Kidney shows vesicular staining of the cytoplasm in PCT epithelial cells. Recommended concentration: 20μg/mL.

Prediction Reactivity

Canine, Rat

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

NP_002075.2

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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