HRH1 Antibody
HRH1 Antibody
Product Specifications
Product Name Alternative
HRH1, histamine receptor H1, HGNC:5182, H1-R, hisH1, histamine receptor, subclass H1, OTTHUMP00000160133, histamine H (1) receptor
UniProt
P35367
Reactivity
Human, Rat
Immunogen
The immunogen for this antibody is: CNENFKKTFKRILH
Target
HRH1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave bands at approx 55kDa and 37kDa in Human Duodenum lysate after 0.3μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the 37kDa band we observe given the calculated size of 55.8kDa according to NP_000852.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional band) .Immunohistochemistry:In paraffin embedded Human Colon shows membranous staining. Recommended concentration: 4-6μg/mL. Paraffin embedded Human Small Intestine. Recommended concentration: 5μg/mL. This product has been successfully used in IHC on Rat (PMID: 30226827) .Immunofluorescence: This product has been successfully used in IF on Rat (PMID: 30413645) .
Prediction Reactivity
Bovine, Canine, Mouse
Tested Applications
ELISA, IF, IHC
NCBI Accession Number
NP_001091683.1, NP_000852.1, NP_001091681.1, NP_001091682.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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