HNF4A Antibody
HNF4A Antibody
Product Specifications
Product Name Alternative
HNF4A, TCF, HNF4, NR2A1, TCF14, HNF4a7, HNF4a8, HNF4a9, NR2A21, hepatocyte nuclear factor 4, alpha, HNF4-alpha, transcription factor-14, FLJ39654, HNF4alpha, MODY, MODY1, HNF4alpha10/11/12, hepatic nuclear factor 4 alpha
UniProt
P41235
Reactivity
Human, Mouse
Immunogen
The immunogen for this antibody is: RLSKTLVDMDMADY-C
Target
HNF4A
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 55kDa and 48kDa bands observed in Mouse Small Intestine and in lysates of cell line HepG2 (calculated MW of 52.8kDa according to Human NP_000448.3 , 46.6kDa according to Human NP_849181.1, 52.7kDa according to Mouse NP_032287.2 and 49.9kDa according to Mouse NP_001299835.1) . Recommended concentration: 0.1-0.5μg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 2μg/mL.
Prediction Reactivity
Canine, Porcine
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_849180.1, NP_849181.1, NP_000448.3
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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