HGS Antibody
HGS Antibody
Product Specifications
Product Name Alternative
HGS, hepatocyte growth factor-regulated tyrosine kinase substrate , HGNC:4897, HRS, ZFYVE8, human growth factor-regulated tyrosine kinase substrate
UniProt
O14964
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-NRNYWEKKQEEARK
Target
HGS
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave bands at approx 140kDa and 26kDa in Human lysates of monocytic leukemia cell line U937 after 0.3μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 86.2kDa according to NP_004703.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) .Immunohistochemistry:In paraffin embedded Human Testis shows strong staining of Leydig cells. Recommended concentration: 1-2μg/mL.
Prediction Reactivity
Canine, Mouse, Rat
Tested Applications
ELISA, IHC
NCBI Accession Number
NP_004703.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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