What is the catalog number for HTATSF1 Antibody?

The catalog number for HTATSF1 Antibody is orb1247377.

What sizes are available for HTATSF1 Antibody?

HTATSF1 Antibody is available in the following size(s): 0.1 mg.

Is HTATSF1 Antibody in stock?

HTATSF1 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

HTATSF1 Antibody

Product Specifications

Product Name Alternative

HTATSF1, HIV-1 Tat specific factor 1 , RP1-196E23.2, TAT-SF1, dJ196E23.2, HIV TAT specific factor 1, cofactor required for Tat activation of HIV-1 transcription

UniProt

O43719

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-QELYGDGKDGDTQTD

Target

HTATSF1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Epigenetics & Chromatin

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 140kDa band observed in lysates of cell line Daudi (calculated MW of 85.9kDa according to NP_055315.2) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Zhou and Sharp, Science. 1996 Oct 25;274 (5287) :605-10.; PMID: 8849451) . Recommended concentration: 1-3μg/mL. An additional band of unknown identity was also consistently observed at 20kDa. This band was successfully blocked by incubation with the immunising peptide.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Prediction Reactivity

Canine, Rat

Tested Applications

ELISA, WB

NCBI Accession Number

NP_055315.2

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.