IRF6 Antibody

Product Specifications

Product Name Alternative

IRF6, interferon regulatory factor 6, LPS, PIT, PPS, VWS, OFC6

UniProt

O14896

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-TPSMQLPPALPPQ

Target

IRF6

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Stem Cell & Developmental Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 60kDa band observed in Human Ovary lysates (calculated MW of 53.1kDa according to NP_006138.1) . In transfected HEK293 transiently expressing IRF6 a band of approx. 55kDa is observed. This band is not observed in the non-transfected HEK293. Recommended for use at 0.2-0.6μg/mL.Immunohistochemistry:In paraffin embedded Human Kidney shows strong nuclear staining in DCT and glomerulus. Recommended concentration: 2-3μg/mL. Paraffin embedded Human Prostate. Recommended concentration: 5μg/mL.

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

NP_006138.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.