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ITK Antibody

ITK Antibody

Product Specifications

Product Name Alternative

ITK, EMT, LYK, PSCTK2, IL2-inducible T-cell kinase, tyrosine-protein kinase LYK, homolog of mouse T-cell itk/tsk, tyrosine-protein kinase ITK/TSK, MGC126257, MGC126258

UniProt

Q08881

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-RLLRQLAEIAESGL

Target

ITK

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Signal Transduction

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:In transfected HEK293 transiently expressing full-length Human ITK (myc and DYKDDDDK tagged), a band of approx. 75kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti- DYKDDDDK tag antibody. Recommended concentration, 1-2μg/mL.

Prediction Reactivity

Mouse

Tested Applications

ELISA, WB

NCBI Accession Number

NP_005537.3

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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