KIF4A Antibody
KIF4A Antibody
Product Specifications
Product Name Alternative
KIF4A, kinesin family member 4A, FLJ12530, FLJ12655, FLJ14204, FLJ20631, HSA271784, KIF4, KIF4-G1, chromokinesin, kinesin family member 4
UniProt
O95239
Reactivity
Human, Mouse
Immunogen
The immunogen for this antibody is: SFFSGCSPIEEEAH
Target
KIF4A
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 150kDa band observed in nuclear lysates of cell line NIH3T3 (calculated MW of 139.5kDa according to Mouse NP_032472.2) . Recommended concentration:1-2μg/mL. Primary incubation 1 hour at room temperature. Negative Control: Human Ovary lysate.Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10μg/mL. Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10μg/mL.
Prediction Reactivity
Bovine, Porcine, Rat
Tested Applications
ELISA, FC, IF, WB
NCBI Accession Number
NP_036442.3
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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