KLF15 Antibody
KLF15 Antibody
Product Specifications
Product Name Alternative
KLF15, KKLF, Kruppel-like factor 15, KKLF protein, kidney-enriched Kruppel-like factor, DKFZp779M1320
UniProt
Q9UIH9
Reactivity
Human
Immunogen
The immunogen for this antibody is: VDHLLPVDENFSS-C
Target
KLF15
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 45kDa band observed in HeLa and in nuclear cell lysates of HepG2 (calculated MW of 44kDa according to NP_054798.1) . Recommended concentration: 0.3-1μg/mL. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat, Mouse and Pig Kidney, and NIH3T3 for this particular batch.Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10μg/mL.
Prediction Reactivity
Mouse, Porcine, Rat
Tested Applications
ELISA, FC, WB
NCBI Accession Number
NP_054798.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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