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KLF15 Antibody

KLF15 Antibody

Product Specifications

Product Name Alternative

KLF15, KKLF, Kruppel-like factor 15, KKLF protein, kidney-enriched Kruppel-like factor, DKFZp779M1320

UniProt

Q9UIH9

Reactivity

Human

Immunogen

The immunogen for this antibody is: VDHLLPVDENFSS-C

Target

KLF15

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 45kDa band observed in HeLa and in nuclear cell lysates of HepG2 (calculated MW of 44kDa according to NP_054798.1) . Recommended concentration: 0.3-1μg/mL. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat, Mouse and Pig Kidney, and NIH3T3 for this particular batch.Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10μg/mL.

Prediction Reactivity

Mouse, Porcine, Rat

Tested Applications

ELISA, FC, WB

NCBI Accession Number

NP_054798.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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