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LHCGR Antibody

LHCGR Antibody

Product Specifications

Product Name Alternative

LHCGR, luteinizing hormone/choriogonadotropin receptor , LCGR, LGR2, LHR, hLHR , luteinizing hormone receptor, lutropin/choriogonadotropin receptor, LH receptor, CG receptor

UniProt

P22888

Reactivity

Human

Immunogen

The immunogen for this antibody is: CQGTALLDKTRYTE

Target

LHCGR

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 35kDa band in K562 cell lysates after 0.1μg/mL antibody staining. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature. Please note that we cannot currently find an explanation in the literature for this band, given the calculated size of 78.6kDa according to NP_000224.2.Immunofluorescence: Strong expression of the protein seen in the plasma membranes of HeLa cells. Recommended concentration: 10μg/mL. Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10μg/mL.

Tested Applications

ELISA, FC, IF

NCBI Accession Number

NP_000224.2

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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