LRP6 Antibody
LRP6 Antibody
Product Specifications
Product Name Alternative
LRP6, low density lipoprotein receptor-related protein 6, low density lipoprotein-related protein 6, ADCAD2, FLJ90062, FLJ90421
UniProt
O75581
Reactivity
Human
Immunogen
The immunogen for this antibody is: CMTSVATAKGYTSDL
Target
LRP6
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments gave bands at approx 50kDa and 35kDa in Human Adipose lysates after 0.1μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 180kDa according to NP_002327.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) .Immunohistochemistry:In paraffin embedded Human Kidney shows luminar membrane staining in DCT. Recommended concentration: 4-6μg/mL.
Prediction Reactivity
Canine, Mouse, Rat
Tested Applications
ELISA, IHC
NCBI Accession Number
NP_002327.2
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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