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MAD2L1 Antibody

MAD2L1 Antibody

Product Specifications

Product Name Alternative

MAD2L1, MAD2 mitotic arrest deficient-like 1 (yeast), MAD2, HSMAD2, mitotic arrest deficient, yeast, homolog-like 1, MAD2 (mitotic arrest deficient, yeast, homolog) -like 1, MAD2-like 1, MAD2-like protein 1

UniProt

Q13257

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-KVNSMVAYKIPVND

Target

MAD2L1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 24kDa band observed in lysates of cell line HEK293 (calculated MW of 23.5kDa according to NP_002349.1) . Recommended concentration: 2-4μg/mL. An additional band of unknown identity was also consistently observed at approx. 29kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Prediction Reactivity

Bovine, Canine, Mouse, Porcine, Rat

Tested Applications

ELISA, WB

NCBI Accession Number

NP_002349.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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