MBD2 Antibody
MBD2 Antibody
Product Specifications
Product Name Alternative
MBD2, methyl-CpG binding domain protein 2 , DKFZp586O0821, DMTase, NY-CO-41
UniProt
Q9UBB5
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-RNDPLNQNKGKPDLN
Target
MBD2
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Metabolism Research
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 48-50kDa band observed in lysates of cell line Jurkat (calculated MW of 43.3kDa according to NP_003918.1) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Tan et al, Mol Cell Biol. 2006 Oct;26 (19) :7224-35.; PMID: 16980624) . Recommended concentration: 0.3-1μg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Colon, Adrenal Gland and Skin. Recommended concentration: 2.5μg/mL.
Prediction Reactivity
Canine, Mouse, Rat
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_003918.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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