What is the catalog number for MIF Antibody?

The catalog number for MIF Antibody is orb1247265.

What sizes are available for MIF Antibody?

MIF Antibody is available in the following size(s): 0.1 mg.

Is MIF Antibody in stock?

MIF Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

MIF Antibody

Product Specifications

Product Name Alternative

MIF, macrophage migration inhibitory factor (glycosylation-inhibiting factor), HGNC:7097, GIF, GLIF, MMIF, glycosylation-inhibiting factor, phenylpyruvate tautomerase, macrophage migration inhibitory factor

UniProt

P14174

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-NAANVGWNNSTFA

Target

MIF

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 13kDa band observed in Human Lymph Node, and Thymus lysates and similar bands are observed in lysates of cell lines Daudi and Jurkat (calculated MW of 12.5kDa according to NP_002406.1) . Recommended concentration: 0.01-0.03μg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Thymus. Recommended concentration: 5μg/mL.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant MIF captured by a rabbit antibody. Recommended reporter concentration: 1.5-3μg/mL

Prediction Reactivity

Mouse, Rat

Tested Applications

EIA, ELISA, IHC, WB

NCBI Accession Number

NP_002406.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.