MSX1 Antibody

Product Specifications

Product Name Alternative

MSX1, HOX7, HYD1, msh homeo box homolog 1 (Drosophila), msh homeobox 1, STHAG1, homeobox 7, msh homeo box 1, msh homeobox homolog 1

UniProt

P28360

Reactivity

Human

Immunogen

The immunogen for this antibody is: TSLPLGVKVEDS-C

Target

MSX1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Stem Cell & Developmental Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments gave an approx. 55-60kDa band in Human Muscle and Heart lysates at 1μg/mL. Further, this band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 31.4kDa according to Human NP_002439.2. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Prostate shows staining of nucleoli in a few epithelial cells. Recommended concentration, 2-4μg/mL. Data obtained from a previous batch (different goat) .

Prediction Reactivity

Bovine, Mouse, Porcine, Rat

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

NP_002439.2

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.