TRIM63 Antibody

Product Specifications

Product Name Alternative

TRIM63, MURF1, MURF-1, RNF28, ring finger protein 28, IRF, SMRZ, MURF2, FLJ32380, iris ring finger protein, muscle specific ring finger protein 2, striated muscle RING zinc finger protein, tripartite motif-containing 63, muscle specific ring finger protein 1

UniProt

Q969Q1

Reactivity

Human

Immunogen

The immunogen for this antibody is: DYKSSLIQDGNPM-C

Target

TRIM63

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Musculoskeletal & Connective Tissue Research

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 60kDa band in Human Skeletal Muscle lysates after 0.1μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 40.2kDa according to NP_115977.2. The 60kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:In paraffin embedded Human Skeletal Muscle shows strong staining in rare nuclei, and weak staining of muscle fibres in longitudinal section. Recommended concentration: 1.25μg/mL.

Prediction Reactivity

Bovine, Canine

Tested Applications

ELISA, IHC

NCBI Accession Number

NP_115977.2

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.