TRIM54 Antibody
TRIM54 Antibody
Product Specifications
Product Name Alternative
TRIM54, MURF-3, tripartite motif-containing 54, RNF30, MURF, ring finger protein 30, muscle-specific RING-finger protein 3, muscle-specific RING-finger protein homolog, OTTHUMP00000122598
UniProt
Q9BYV2
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-GSAGPEEERPDGP
Target
TRIM54
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Stem Cell & Developmental Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Experiments gave bands at approx 45kDa and 38kDa in Human Skeletal Muscle lysate after 1μg/mL antibody staining. These bands correspond to the reported isoforms with calculated MWs of 43.1kDa according to NP_115935, and 38.6kDa according to NP_912730. Recommended concentration: 1-3μg/mL.Immunohistochemistry:Paraffin embedded Human Skeletal Muscle. Recommended concentration: 3.75μg/mL.
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_912730.2, NP_115935.3
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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