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MYF5 Antibody

MYF5 Antibody

Product Specifications

Product Name Alternative

MYF5 , myogenic factor 5

UniProt

P13349

Reactivity

Human

Immunogen

The immunogen for this antibody is: PECNSPVWSRKSST

Target

MYF5

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Stem Cell & Developmental Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 28kDa band observed in Human Skeletal Muscle lysates (calculated MW of 28.4kDa according to NP_005584.1) . Recommended concentration: 0.5-1.5μg/mL. An additional band of 26kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.

Tested Applications

ELISA, WB

NCBI Accession Number

NP_005584.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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