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NANOS1 Antibody

NANOS1 Antibody

Product Specifications

Product Name Alternative

NANOS1, nanos homolog 1 (Drosophila), NOS1, nanos homolog 1

UniProt

Q8WY41

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-SARDGPPGKKLR

Target

NANOS1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 35-37kDa band observed in Human Testis lysates (calculated MW of 30.2kDa according to NP_955631.1) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Clarke et al, Hum Mol Genet. 2004 Apr 1;13 (7) :727-39. Epub 2004 Feb 12, PMID: 14962983) . Recommended concentration: 1-3μg/mL. Primary incubation was 1 hour. This antibody has been successfully used in WB on Human: Sajek et al (2018) PMID: 30269240.

Tested Applications

ELISA, WB

NCBI Accession Number

NP_955631.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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