NANOS1 Antibody
NANOS1 Antibody
Product Specifications
Product Name Alternative
NANOS1, nanos homolog 1 (Drosophila), NOS1, nanos homolog 1
UniProt
Q8WY41
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-SARDGPPGKKLR
Target
NANOS1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 35-37kDa band observed in Human Testis lysates (calculated MW of 30.2kDa according to NP_955631.1) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Clarke et al, Hum Mol Genet. 2004 Apr 1;13 (7) :727-39. Epub 2004 Feb 12, PMID: 14962983) . Recommended concentration: 1-3μg/mL. Primary incubation was 1 hour. This antibody has been successfully used in WB on Human: Sajek et al (2018) PMID: 30269240.
Tested Applications
ELISA, WB
NCBI Accession Number
NP_955631.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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