NDRG1 Antibody
NDRG1 Antibody
Product Specifications
Product Name Alternative
NDRG1, N-myc downstream regulated gene 1, CAP43, , CMT4D, DRG1, GC4, HMSNL, NDR1, NMSL, PROXY1, RIT42, RTP, TARG1, TDD5, N-myc downstream regulated gene 1 protein, differentiation-related gene 1 protein, nickel-specific induction protein Cap43, protein regulated by oxygen-1, reducing agents and tunicamycin-responsive protein, tunicamycin-responsive protein
UniProt
Q92597
Reactivity
Human
Immunogen
The immunogen for this antibody is: GNSAGPKSMEVSC
Target
NDRG1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Neuroscience
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 48kDa band observed in Human Kidney, and approx 50kDa band observed in Human Brain (Cerebellum) and Testis lysates (calculated MW of 42.8kDa according to NP_006087.2) . The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3μg/mL. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human hepatocelluar carcinoma shows strong labeling of membranes and weaker staining of cytoplasm. These observations are consistent with those obtained by different antibodies. Recommended concentration: 0.5-2μg/mL. Paraffin embedded Human Prostate and Colon. Recommended concentration: 2.5μg/mL.
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_006087.2
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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