NEDD1 Antibody
NEDD1 Antibody
Product Specifications
Product Name Alternative
NEDD1, neural precursor cell expressed, developmentally down-regulated 1
UniProt
Q8NHV4
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-NQTRNSEKFEKPEN
Target
NEDD1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 75kDa band observed in lysates of cell line HeLa (calculated MW of 72.0kDa according to NP_690869.1) . Recommended concentration: 1-3μg/mL. An additional band was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunising peptide and likely represents the mature form. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Tested Applications
ELISA, WB
NCBI Accession Number
NP_690869.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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