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NEDD1 Antibody

NEDD1 Antibody

Product Specifications

Product Name Alternative

NEDD1, neural precursor cell expressed, developmentally down-regulated 1

UniProt

Q8NHV4

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-NQTRNSEKFEKPEN

Target

NEDD1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 75kDa band observed in lysates of cell line HeLa (calculated MW of 72.0kDa according to NP_690869.1) . Recommended concentration: 1-3μg/mL. An additional band was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunising peptide and likely represents the mature form. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Tested Applications

ELISA, WB

NCBI Accession Number

NP_690869.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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